BioLector XT Microbioreactor
Microfluidic Bioprocess Control
Cultivation conditions
| TEMPERATURE |
| 10 – 50 °C (min. temp.: 8 °C below ambient temp.) |
| SHAKING SPEED |
| 100 – 1500 rpm (3 mm diameter) |
| ENVIRONMENTAL CONDITIONS |
| Active humidification |
| Ambient air |
| 1 – 100 % O2 (optional) |
| 0 – 12 % CO2 (optional) |
| Anaerobic cultivation (optional) |
| OXYGEN OPTODES |
| 0 – 100 % dissolved oxygen*1 |
| pH OPTODES |
| pH 4 – 7.5 (depending on plate) |
| MTP READING TIME |
| 2.7 min / filter / 48 wells @ 1000 rpm |
Microfluidic features*2
| TRIGGERED pH CONTROL (CLOSED LOOP CONTROLLER) |
| pH control range: 4.0 – 7.5 (depending on plate) |
| Fully editable PI control |
| Slow, medium and fast PI default settings |
| FEEDING OPTIONS |
| Two sided pH control (alkali and acid) |
| One sided pH control and one feed line (alkali or acid + one feed) |
| Two feed lines |
| FEEDING PROFILES |
| Profile equation: dV/dt =A+B×t+C×eD×t |
| Constant: A [μL/h] |
| Linear: A [μL/h] and B [μL/h²] |
| Exponential: A [μL/h], B [μL/h²], C [μL/h] and D [h-1] |
| Pulse feed |
| Full feeding profile flexibility permits broad experimental design |
| PUMP RATE |
| Up to 665 pump strokes per hour |
System dimensions
Gassing lid dimensions
Available optional modules
|
Part no. |
Module description | Additional feature |
Note |
| E-XTMF |
TradeGothicLT-Extended |
Active control of pH according to online signals and continuous feeding of up to two solutions | only with Microfluidic plates |
| E-O2XT-100 |
O2 up-regulation module |
Control of gas atmosphere in head space: 21 – 100 % O2 |
|
| E-O2XT-25 |
O2 down-regulation module |
Control of gas atmosphere in head space: 1 – 21 % O2 |
use only with N2 |
| E-CO2XT-12 |
CO2 up-regulation module |
Control of gas atmosphere in head space: 0 – 12 % CO2 |
|
| E-AN-300 |
Anaerobic cultivation module |
Gassing with 100 % N2 allows cultivation of organisms in anaerobic conditions |
use only with N2 |
| All optional modules compatible in one BioLector microbioreactor device. |
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Microtiter plates
| FLOWERPLATE |
| 48 cultivation wells |
| Filling volume: 800 – 1900 μL (rpm dependent) |
| High OTR and high kLa |
| ROUND WELL PLATE |
| 48 cultivation wells |
| Filling volume: 1000 – 2400 μL (rpm dependent) |
| Lower OTR and low shear force |
| MICROFLUIDIC PLATE |
| Available as both FlowerPlate and Round Well Plate |
| 32 cultivation wells controlled by 16 reservoir wells |
| Maximum filling volumes in reservoir wells: 1800 µL (FlowerPlate) and 2000 µL (Round Well Plate) |
| Same filling volumes for cultivation wells as in 48 well plate |
Lab space and material requirements
| Flat surface with a minimal loading capacity of 100 kg for BioLector XT microbioreactor or 250 kg for BioLector XT microbioreactor with the valve control unit*2 |
| Device weight: 58 kg for BioLector XT microbioreactor (61 kg with microfluidic module) and 44 kg for valve control unit*2 |
| 1x power supply for BioLector XT microbioreactor: 90-264 VAC, 47~63 Hz 1x power supply for laptop: 90-230 VAC, 50/60 Hz 1x power supply for valve control unit*2: 90-264 VAC, 47~63 Hz (US/Canada); 85-264 VAC, 47~63 Hz (EU, ROW) |
| Microfluidics: requires 4 to 6 bar dry and oil-free compressed air, 6 mm ØOUT push-in connection |
| Gassing modules (O2 up, O2 down, CO2 up, anaerobic module): require 1.5-2 bar dry and oil-free O2, CO2, or N2; 4 mm ØOUT push-in connection |
| Humidity control: 400 mL deionized water |
*1 100 % corresponding to the DO level reached while gassing with 100 % O2 without O2 consumption
*2 only with optional microfluidic module OTR: Oxygen transfer rate [mmol/L/h]
kLa: Volumetric oxygen transfer coefficient [h-1]
m2p and the m2p logo are the trademarks or registered trademarks of m2p-labs GmbH in the US and other countries.
m2p-labs is a Beckman Coulter company.
Additional BioLector XT Content
